INIFTA   05425
INSTITUTO DE INVESTIGACIONES FISICO-QUIMICAS TEORICAS Y APLICADAS
Unidad Ejecutora - UE
artículos
Título:
Functional nicotinic acetylcholine receptor reconstitution in Au(111)-supported Thiolipid Monolayers
Autor/es:
DIEGO PISSINIS; CAROLINA DIAZ; ELIANA MAZA; IDA C. BONINI; FRANCISCO C. BARRANTES; ROBERTO C. SALVAREZZA; PATRICIA L. SCHILARDI
Revista:
Nanoscale
Editorial:
Royal Chemistry Society
Referencias:
Lugar: CAMBRIDGE; Año: 2015 vol. 7 p. 15789 - 15797
ISSN:
2040-3364
Resumen:
The insertion and function of the muscle-type nicotinic acetylcholine receptor (nAChR) in Au(111)- supported thiolipid self-assembled monolayers have been studied by atomic force microscopy (AFM), surface plasmon resonance (SPR), and electrochemical techniques. It was possible for the first time to resolve the supramolecular arrangement of the protein spontaneously inserted in a thiolipid monolayer in an aqueous solution. Geometric supramolecular arrays of nAChRs were observed, most commonly in a triangular form compatible with three nAChR dimers of ∼20 nm each. Addition of the full agonist carbamoylcholine activated and opened the nAChR ion channel, as revealed by the increase in capacitance relative to that of the nAChR?thiolipid system under basal conditions. Thus, the self-assembled system appears to be a viable biomimetic model to measure ionic conductance mediated by ion-gated ion channels under different experimental conditions, with potential applications in biotechnology and pharmacology