Influence of nano-microstructure of the surface on bacterial adhesion

DÍAZ, C; CORTIZO MC,; SCHILARDI, P; GÓMEZ DE SARAVIA, G; FERNÁNDEZ LORENZO DE MELE, M

MATERIALS RESEARCH

Scielo

Año: 2007 vol. 10 p. 11 - 14

1516-1439

NOTA: todos los autores han contribuido de igual manera a la elaboración de este trabajo.Biomaterials failures are frequently associated to the formation of bacterial biofilms on the surface. The aim of this work is to study the adhesion of non motile bacteria streptococci consortium and motile Pseudomonas fluorescens. Substrates with micro and nanopatterned topography were used. The influence of surface characteristics on bacterial adhesion was investigated using optical and epifluorescence microscopy, scanning electron microscopy(SEM) and atomic force microscopy (AFM). Results showed an important influence of the substratum nature. On microrough surfaces, initial bacterial adhesion was less significant than on smooth surfaces. In contrast, nanopatterned samples showed more bacterial attachment than the smooth control. It was also noted a remarkable difference in morphology, orientation and distribution of bacteria between the smooth and the nanostructured substrate. The results show the important effect of substratum nature and topography on bacterial adhesion whichdepended on the relation between roughness characteristics dimensions and bacterial size.