INIFTA   05425
INSTITUTO DE INVESTIGACIONES FISICO-QUIMICAS TEORICAS Y APLICADAS
Unidad Ejecutora - UE
artículos
Título:
ANTIOXIDANT ACTIVITY OF CONJUGATED LINOLEIC ACID ISOMERS, LINOLEIC ACID AND ITS METHYL ESTER DETERMINED BY PHOTOEMISSION AND DPPH TECHNIQUES
Autor/es:
FAGALI, N. S.; ANGEL CATALA
Revista:
Biophysical Chemistry
Editorial:
Elsevier
Referencias:
Lugar: The Netherlands; Año: 2008 vol. 137 p. 56 - 62
ISSN:
0301-4622
Resumen:
The chemiluminescent response of conjugated linoleic acid isomers (CLAs), linoleic acid (LA) and methyl
linoleate (LAME) against the prooxidant t-butyl hydroperoxide (tBHP) was analyzed. The c9, t11-CLA and t10,
c12-CLA isomers showed significant photoemission at the highest concentration used, while photoemission
was not detected at any concentration of LA and LAME analyzed. These results show that CLAs are more
susceptible to peroxidation than LA and LAME. Likewise, the effect of CLA, LA and LAME on lipid peroxidation
of triglycerides rich in C20:5 ù3 and C22:6 ù3 (Tg ù3-PUFAs) was investigated. For that, chemiluminescence
produced by triglycerides in the presence of tBHP, previously incubated with different concentrations of
CLAs, LA and LAME (from 1 to 200 mM) was registered for 60 min. Triglycerides in the presence of t-BHP
produced a peak of light emission (3151±134 RLUs) 5 min after addition. CLAs produced significant inhibition
on photoemission, t10, c12-CLA being more effective than the c9, t11-CLA isomer. LA and LAME did not have
an effect on lipid peroxidation of Tg ù3-PUFAs.
CLA isomers, LA and LAME were also investigated for free radical scavenging properties against the stable
radical (DPPHU). Both CLA isomers reacted and quenched DPPHUat all tested levels (from 5 to 25 mM), while
LA and LAME did not show radical quenching activity even at the highest concentration tested. These data
indicate that CLAs would provide protection against free radicals, but LA and LAME cannot.t-butyl hydroperoxide (tBHP) was analyzed. The c9, t11-CLA and t10,
c12-CLA isomers showed significant photoemission at the highest concentration used, while photoemission
was not detected at any concentration of LA and LAME analyzed. These results show that CLAs are more
susceptible to peroxidation than LA and LAME. Likewise, the effect of CLA, LA and LAME on lipid peroxidation
of triglycerides rich in C20:5 ù3 and C22:6 ù3 (Tg ù3-PUFAs) was investigated. For that, chemiluminescence
produced by triglycerides in the presence of tBHP, previously incubated with different concentrations of
CLAs, LA and LAME (from 1 to 200 mM) was registered for 60 min. Triglycerides in the presence of t-BHP
produced a peak of light emission (3151±134 RLUs) 5 min after addition. CLAs produced significant inhibition
on photoemission, t10, c12-CLA being more effective than the c9, t11-CLA isomer. LA and LAME did not have
an effect on lipid peroxidation of Tg ù3-PUFAs.
CLA isomers, LA and LAME were also investigated for free radical scavenging properties against the stable
radical (DPPHU). Both CLA isomers reacted and quenched DPPHUat all tested levels (from 5 to 25 mM), while
LA and LAME did not show radical quenching activity even at the highest concentration tested. These data
indicate that CLAs would provide protection against free radicals, but LA and LAME cannot.ficant photoemission at the highest concentration used, while photoemission
was not detected at any concentration of LA and LAME analyzed. These results show that CLAs are more
susceptible to peroxidation than LA and LAME. Likewise, the effect of CLA, LA and LAME on lipid peroxidation
of triglycerides rich in C20:5 ù3 and C22:6 ù3 (Tg ù3-PUFAs) was investigated. For that, chemiluminescence
produced by triglycerides in the presence of tBHP, previously incubated with different concentrations of
CLAs, LA and LAME (from 1 to 200 mM) was registered for 60 min. Triglycerides in the presence of t-BHP
produced a peak of light emission (3151±134 RLUs) 5 min after addition. CLAs produced significant inhibition
on photoemission, t10, c12-CLA being more effective than the c9, t11-CLA isomer. LA and LAME did not have
an effect on lipid peroxidation of Tg ù3-PUFAs.
CLA isomers, LA and LAME were also investigated for free radical scavenging properties against the stable
radical (DPPHU). Both CLA isomers reacted and quenched DPPHUat all tested levels (from 5 to 25 mM), while
LA and LAME did not show radical quenching activity even at the highest concentration tested. These data
indicate that CLAs would provide protection against free radicals, but LA and LAME cannot.ù3 and C22:6 ù3 (Tg ù3-PUFAs) was investigated. For that, chemiluminescence
produced by triglycerides in the presence of tBHP, previously incubated with different concentrations of
CLAs, LA and LAME (from 1 to 200 mM) was registered for 60 min. Triglycerides in the presence of t-BHP
produced a peak of light emission (3151±134 RLUs) 5 min after addition. CLAs produced significant inhibition
on photoemission, t10, c12-CLA being more effective than the c9, t11-CLA isomer. LA and LAME did not have
an effect on lipid peroxidation of Tg ù3-PUFAs.
CLA isomers, LA and LAME were also investigated for free radical scavenging properties against the stable
radical (DPPHU). Both CLA isomers reacted and quenched DPPHUat all tested levels (from 5 to 25 mM), while
LA and LAME did not show radical quenching activity even at the highest concentration tested. These data
indicate that CLAs would provide protection against free radicals, but LA and LAME cannot.t-BHP
produced a peak of light emission (3151±134 RLUs) 5 min after addition. CLAs produced significant inhibition
on photoemission, t10, c12-CLA being more effective than the c9, t11-CLA isomer. LA and LAME did not have
an effect on lipid peroxidation of Tg ù3-PUFAs.
CLA isomers, LA and LAME were also investigated for free radical scavenging properties against the stable
radical (DPPHU). Both CLA isomers reacted and quenched DPPHUat all tested levels (from 5 to 25 mM), while
LA and LAME did not show radical quenching activity even at the highest concentration tested. These data
indicate that CLAs would provide protection against free radicals, but LA and LAME cannot.ficant inhibition
on photoemission, t10, c12-CLA being more effective than the c9, t11-CLA isomer. LA and LAME did not have
an effect on lipid peroxidation of Tg ù3-PUFAs.
CLA isomers, LA and LAME were also investigated for free radical scavenging properties against the stable
radical (DPPHU). Both CLA isomers reacted and quenched DPPHUat all tested levels (from 5 to 25 mM), while
LA and LAME did not show radical quenching activity even at the highest concentration tested. These data
indicate that CLAs would provide protection against free radicals, but LA and LAME cannot.ù3-PUFAs.
CLA isomers, LA and LAME were also investigated for free radical scavenging properties against the stable
radical (DPPHU). Both CLA isomers reacted and quenched DPPHUat all tested levels (from 5 to 25 mM), while
LA and LAME did not show radical quenching activity even at the highest concentration tested. These data
indicate that CLAs would provide protection against free radicals, but LA and LAME cannot.U). Both CLA isomers reacted and quenched DPPHUat all tested levels (from 5 to 25 mM), while
LA and LAME did not show radical quenching activity even at the highest concentration tested. These data
indicate that CLAs would provide protection against free radicals, but LA and LAME cannot.