INIFTA   05425
INSTITUTO DE INVESTIGACIONES FISICO-QUIMICAS TEORICAS Y APLICADAS
Unidad Ejecutora - UE
artículos
Título:
Bacterial biofilms formed "in vitro" and "in vivo" on orthodontic appliances. Effect of antimicrobial agents
Autor/es:
CORTIZO MC,; LAGARES, ME; FERNÁNDEZ LORENZO DE MELE, M
Revista:
Revista CENIC
Editorial:
CENIC Ciencias Biológicas
Referencias:
Lugar: La Habana; Año: 2006 vol. 37 p. 159 - 161
ISSN:
0258 6002
Resumen:
Cariogenic processes and peri-implant and gingival infections can occur as a consequence of the biofilm formation. In order to prevent these processes, prophylactic products like sodium fluoride (F), chlorhexidine (C) and xylitol (X) are used. The aim of this work was to study the efect of commercially available mouth rinses containing F + X and C+ X on oral biofilms formed on orthodontic appliances. A consortium of streptococci was used in the in vitro experiments and Agar Mitis Salivarious and its modified composition for liquid cultures. Sainless steel and elastic bands were immersed in the inoculated culture media for seven days and the growth of biofilms was followed in real time through optical microscopy made in situ. The sessile bacteria attached on the bands were also observed using epifluorescence microscopy after staining the sample with acridine orange. Subsequent to the preset periods the biofilmed bands were removed from the culture medium and were transferrewd to three different flasks with: i) a prosphate buffer, ii) a mouth rinse containing F (o.o5 %) + X (10 %), iii) a mouth rinse containing X (10 %) + C (0.12%). Results demonstrated that it is difficult to predict the efficacy of an AA aganinst oral biofilms based on experiments performed with planktonic cells. The assayed AA were able to diffuse into the biofilm, change the microstructural characteristics of the biofilm which becomes more compact, slow down the growth of the sessile bacteria and promote the detachment of cells. However, re-growth of the biofilm could occur under better environmental conditions when the treatment finishes.