INIFTA   05425
INSTITUTO DE INVESTIGACIONES FISICO-QUIMICAS TEORICAS Y APLICADAS
Unidad Ejecutora - UE
artículos
Título:
In vitro degradation and biological effects of magnesium particles on osteoblastic cells. Influence of fluoride treatments
Autor/es:
- GRILLO, C.A, F. ALVAREZ AND M. FERNÁNDEZ LORENZO DE MELE
Revista:
COLLOIDS AND SURFACES B-BIOINTERFACES
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2011 vol. 88 p. 471 - 476
ISSN:
0927-7765
Resumen:
Mg-based materials are promising for orthopedic, dental, and cardiovascular applications. However, their high degradation rate in vivo (release of Mg ions and debris particles) is cause of great concern. Protective treatments involving the generation of fluoride conversion coatings have been proposed in order to reduce corrosion rates. The aim of this study was to evaluate Mg debris biodegradation and its possible cytotoxic effects on osteoblastic cells in situ.  Neutral red dying and Acridine Orange staining techniques were used as endpoints to analyze the cytotoxic effects at 100-2000 µg/mL concentration range. Results showed a marked variation of Mg ion concentration in the culture medium after different exposure periods (1, 2, or 24h). Interestingly, the release rate of magnesium particles (MP) with and without fluoride treatment was dependent on the presence or absence of cells. Adverse effects induced by >1000 µg/mL MP doses and Mg ion concentrations higher than 480 µg/mL were observed on cells. Results showed significant  differences between the concentration of Mg ion in the presence and absence of cells. This fact reveals a dynamic equilibrium mediated by Mg ion input and output in the cells that leads to the change in MP corrosion rates. Fluoride release from conversion coatings did not show cytotoxic effects.