ANTIOXIDANT ENZYME ACTIVITY AND OXIDATIVE STRESS IN POMACEA CANALICULATA (MOLLUSCA: GASTROPODA) EXPOSED TO CYPERMETHRIN

LAVARIAS, SABRIA MARIA LUISA; AMBROSIO, EUGENIA; ASTIZ, MARIANA; ARRIGHETTI, FLORENCIA; GARDA, HORACIO; RODRIGUES CAPÍTULO, ALBERTO

Buenos Aires

Congreso; XI Biennial Meeting SETAC Latinoamérica; 2015

Sociedad de Toxicología y Química Ambiental

Several agrochemicals extensively used to control pests in agricultural practices could affect non-target fauna. Most pesticides are lipophilic and passively absorbed through lipid membranes. The effect of these compounds in organisms depends on their susceptibility to biotransformation. In certain organisms, pesticides could alter reactions in aerobic oxidative metabolic pathways causing oxidative stress, condition that leads to protein degradation and enzyme inactivation, lipid peroxidation, damage of nucleic acids and finally cell death. In Argentina, the pyrethroid cypermethrin (CYP) is widely used in agricultural activities. In order to determine metabolic disorders that could be used as biomarkers of pollution, the native freshwater snail Pomacea canaliculata was selected for the present study. Adult males and females of snails were exposed to sublethal CYP concentrations (10, 25 and 100 µg /L) for 1, 4, 7 and 14 days and a biodepuration assay was also performed. The activities of the enzymes superoxide dismutase (SOD), catalase (CAT) and glutathione-S-transferase (GST) and the levels of lipid peroxidation (LPO) and protein oxidation (PO) in digestive gland and gills were assessed. In digestive gland, CYP caused a significant increase in the antioxidant enzymes SOD, CAT and GST activity compared to the control group (p