Parametric and surface science investigation of the enzimatic resolution of Ibuprofen with Novozym 435

CARLA JOSÉ; LAURA E. BRIAND

Washington, EEUU

Congreso; 238th American Chemical Society National Meeting and Exposition; 2009

American Chemical Society

Ibuprofen is a nonsteroidal anti-inflammatory pharmaceutical of massive use. Although nowadays most commercially available medicines contain the racemic mixture of the pharmaceutical, it is well documented that the therapeutic activity of ibuprofen is attributed to the (S)-enantiomer.  With the aim of increasing the fraction of the active enantiomer in the pharmaceutical in this contribution we analyze the feasibility of the enzymatic resolution of racemic ibuprofen catalyzed with Novozym 435 a commercial immobilized version of Candida antarctica B lipase. The surface interaction of ibuprofen and ethanol with the biocatalyst has been investigated through conventional adsorption isotherms and temperature programmed surface reaction (TPSR). The results gave evidences of the strong adsorption of ethanol on the biocatalyst which might account for its inhibitory effect under reaction conditions. Moreover the adsorption of ibuprofen at 301 K allowed determining the number of surface active sites Ns.