CINDECA   05422
CENTRO DE INVESTIGACION Y DESARROLLO EN CIENCIAS APLICADAS "DR. JORGE J. RONCO"
Unidad Ejecutora - UE
artículos
Título:
Influence of the nature of the support in the catalytic performance of CALB: experimental and theoretical evidences
Autor/es:
PAULA NICOLÁS; LAURA E BRIAND; MARÍA VICTORIA TOLEDO; MARÍA LUJÁN FERREIRA; CARLA JOSÉ; VERÓNICA L. LASSALLE
Revista:
Catalysis Science and Technology
Editorial:
Royal Society of Chemistry
Referencias:
Año: 2018 vol. 8 p. 3513 - 3513
ISSN:
2044-4753
Resumen:
The immobilization of the lipase B of Candida antarctica (CALB) was carried out on various hydrophilic and hydrophobic supports through adsorption and covalent bond interactions. The catalytic performance of the biocatalysts was evaluated in the enantioselective esterification the R/S-ibuprofen with ethanol without co-solvents added. The present investigation demonstrates that the biocatalysts based on CALB immobilized by adsorption on hydrophobic supports (acrylic resin,polymethylmethacrylate, polystyrene, epoxy resin and polypropylene) possesshigher conversion of ibuprofen and enantiomeric excess towards the S-enantiomerthan CALB immobilized onto hydrophilic materials. Additionally, the enzyme loading is a key factor influencing the conversion of the substrate regardless the nature of the support material. High enzyme loading is detrimental for the specific activity of the biocatalysts. Experimental and molecular modeling investigations provided evidences of the influence of the water activity in the stereospecificity of the lipase.
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