Influence of the nature of the support in the catalytic performance of CALB: experimental and theoretical evidences

PAULA NICOLÁS; LAURA E BRIAND; PAULA NICOLÁS; LAURA E BRIAND; MARÍA VICTORIA TOLEDO; MARÍA LUJÁN FERREIRA; MARÍA VICTORIA TOLEDO; MARÍA LUJÁN FERREIRA; CARLA JOSÉ; VERÓNICA L. LASSALLE; CARLA JOSÉ; VERÓNICA L. LASSALLE

Catalysis Science and Technology

Royal Society of Chemistry

Año: 2018 vol. 8 p. 3513 - 3526

2044-4753

The immobilization of the lipase B of Candida antarctica (CALB) was carried out on various hydrophilic and hydrophobic supports through adsorption and covalent bond interactions. The catalytic performance of the biocatalysts was evaluated in the enantioselective esterification the R/S-ibuprofen with ethanol without co-solvents added. The present investigation demonstrates that the biocatalysts based on CALB immobilized by adsorption on hydrophobic supports (acrylic resin,polymethylmethacrylate, polystyrene, epoxy resin and polypropylene) possesshigher conversion of ibuprofen and enantiomeric excess towards the S-enantiomerthan CALB immobilized onto hydrophilic materials. Additionally, the enzyme loading is a key factor influencing the conversion of the substrate regardless the nature of the support material. High enzyme loading is detrimental for the specific activity of the biocatalysts. Experimental and molecular modeling investigations provided evidences of the influence of the water activity in the stereospecificity of the lipase.