CENTRO DE INVESTIGACION Y DESARROLLO EN CIENCIAS APLICADAS "DR. JORGE J. RONCO"
Unidad Ejecutora - UE
A mixture of kefir-isolated bacteria and yeasts inhibits Shigella flexneri invasion and modulates the pro-inflammatory response on intestinal epithelial cells
BOLLA, P. A.; ABRAHAM A.G; PEREZ, P.; SERRADELL, M. DE LOS A.
Wageningen Academic Publishers
Año: 2016 vol. 1 p. 103 - 103
The aim of this work was to evaluate the ability of a kefir-isolated microbial mixture containing three bacterial and two yeast strains (MM) to protect intestinal epithelial cells against Shigella flexneri invasion, as well as to analyse the effect on pro-inflammatory response elicited by this pathogen. A significant decrease in S. flexneri strain 72 invasion was observed on both HT-29 and Caco-2 cells pre-incubated with MM. Pre-incubation with the individual strains Saccharomyces cerevisiae CIDCA 8112, or Lactococcus lactis subp lactis CIDCA 8221 also reduced the internalization of S. flexneri into HT-29 cells although in a lesser extent than MM. Interestingly, Lactobacillus plantarum CIDCA 83114 exerted a protective effect on the invasion of Caco-2 and HT-29 cells by S. flexneri. Regarding pro-inflammatory response on HT-29 cells, S. flexneri infection induced a significant activation of the expression of IL-8, CCL20 and TNF-?Ñ encoding genes (P