Biotransformation of 2,6-diaminopurine nucleosides by immobilized Geobacillus stearothermophilus

E. C. DE BENEDETTI; C. W. RIVERO; C. N. BRITOS; M. E. LOZANO; J. A. TRELLES

BIOTECHNOLOGY PROGRESS

AMER CHEMICAL SOC

Lugar: Washington; Año: 2012 vol. 28 p. 1251 - 1256

8756-7938

An efficient and green bioprocess to obtain 2,6-diaminopurine nucleosides using thermophilic bacteria is herein reported. Geobacillus stearothermophilus CECT 43 showed a conversion rate of 90 and 83% at 2 h to obtain 2,6-diaminopurine-2´-deoxyriboside and 2,6-diaminopurine riboside, respectively. The selected biocatalyst was successfully stabilized in an agarose matrix and used to produce up to 23.4 g of 2,6-diaminopurine-2´-deoxyriboside in 240 h of process. These nucleoside analogues can be used as prodrug precursors or in antisense oligonucleotide synthesis.