CINDECA   05422
CENTRO DE INVESTIGACION Y DESARROLLO EN CIENCIAS APLICADAS "DR. JORGE J. RONCO"
Unidad Ejecutora - UE
artículos
Título:
Biotransformation of 2,6-diaminopurine nucleosides by immobilized Geobacillus stearothermophilus
Autor/es:
E. C. DE BENEDETTI; C. W. RIVERO; C. N. BRITOS; M. E. LOZANO; J. A. TRELLES
Revista:
BIOTECHNOLOGY PROGRESS
Editorial:
AMER CHEMICAL SOC
Referencias:
Lugar: Washington; Año: 2012 vol. 28 p. 1251 - 1251
ISSN:
8756-7938
Resumen:
An efficient and green bioprocess to obtain 2,6-diaminopurine nucleosides using thermophilic bacteria is herein reported. Geobacillus stearothermophilus CECT 43 showed a conversion rate of 90 and 83% at 2 h to obtain 2,6-diaminopurine-2´-deoxyriboside and 2,6-diaminopurine riboside, respectively. The selected biocatalyst was successfully stabilized in an agarose matrix and used to produce up to 23.4 g of 2,6-diaminopurine-2´-deoxyriboside in 240 h of process. These nucleoside analogues can be used as prodrug precursors or in antisense oligonucleotide synthesis.
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