Biosynthesis of anti-HCV compounds using thermophilic microorganisms

RIVERO C. W. ; DE BENEDETTI E.C.; SAMBETH J.E.; LOZANO M.E. ; TRELLES J.A.

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

PERGAMON-ELSEVIER SCIENCE LTD

Lugar: Amsterdam; Año: 2012 vol. 22 p. 6059 - 6062

0960-894X

This work describes the application of thermophilic microorganisms for obtaining 6-halogenated purine nucleosides. Biosynthesis of 6-chloropurine-20-deoxyriboside and 6-chloropurine riboside was achieved by Geobacillus stearothermophilus CECT 43 with a conversion of 90% and 68%, respectively. Furthermore, the selected microorganism was satisfactorily stabilized by immobilization in an agarose matrix. This biocatalyst can be reused at least 70 times without significant loss of activity, obtaining 379 mg/L of 6- chloropurine-20-deoxyriboside. The obtained compounds can be used as antiviral agents.