FTIR, SEM and Fractal dimension characterization of of Lipase B from Candida Antactica B Immobilized onto Titania at Selected Conditions

MARIA LAURA FORESTI; GRACIELA M. VALLE; RITA D. BONETTO; MARÍA LUJAN FERREIRA; LAURA ESTEFANÍA BRIAND

APPLIED SURFACE SCIENCE

Elsevier

Lugar: North Holland; Año: 2010 vol. 256 p. 1624 - 1635

0169-4332

Lipase B from Candida antarctica (CALB) was immobilized onto titanium dioxide (TiO2). The adsorption isotherm was determined by UV-VIS analysis of supernatant solution at 280 nm, revealing that in seven hours 98% of the theoretic lipase monolayer was achieved. Samples withdrawn from the supernatant immobilization medium were analyzed by Fourier-transform infrared spectroscopy in the 1700 to 1600 cm-1 range (Amide I) in order to obtain quantitative information on the evolution of the secondary-structure elements that compose the protein. The analysis performed revealed that lipase conformation suffers only minor changes during its adsorption onto TiO2. Characterization of the immobilized biocatalyst (CALB/TiO2) implied SEM, AFM, fractal dimension analysis and FTIR techniques.