The impact of aerobic training in cardiovascular alterations in ovariectomized rats.

GODOY COTO J; ENNIS IL; YEVES AM; AIELLO EA; IBAÑEZ AM; VELEZ RUEDA O; DE GIUSTI VC

Mar del Plata

Congreso; Reunión de Sociedades de Biociencias 2020. Reunión Anual de la Sociedad Argentina de Fisiología (SAFIS); 2020

Menopausal women show an increase in cardiovascular diseases. Sodium/proton exchanger (NHE) and sodium/bicarbonate cotransporter (NBC) are the main alkalinizing mechanisms of the cardiomyocytes. Last year we reported that the NHE activity is increased, whereas the NBCe1 is decreased in ovariectomized (OVX) rats. Ennis et al. have demonstrated that an aerobic routine is able to transform a pathological cardiac hypertrophy (CH) to a physiological one, possibly related to an inhibition of the NHE activity. Additionally, it was described an increase in NBCe1 activity in mice after a swimming routine. Here we test the hypothesis that swimming is able to prevent the cardiovascular modifications previously described by our team in OVX rats. Bilateral ovariectomy was performed in 10 weeks old Wistar rats. OVX rats were randomly assigned to a sedentarygroup (OVXs) or to an aerobic swimming routine (8 weeks/5 d a week) (OVXe). Body weight and visceral adipose tissue were increased in OVXs rats, and both changes were prevented by the swimming training. Left ventricular mass index (echocardiography) was higher in OVX rats in comparison with Sham. (LVMI (mg/mm) Sham 20.14±2.35 n=2; OVXs 23.99±2.17, n=5; OVXe 26.94±4.53 n=4). Ongoing experiments such as fibrosis, capillary density and molecular markerswill determine the type of CH. NHE activity was increased in OVXs myocytes, and swimming showed a tendency to prevent it (JH at pH=6.8 (mmol/min) Sham 1.63±0.28 n=4; OVXs 2.04±0.18, n=3; OVXe 1.73±0.28 n=4). NBCe1 activity was diminished in OVXs myocytes, whereas OVXerecovered the impaired activity (DpH= Sham: 0.25±0.02, n=4; OVXs 0.10±0.02*, n=8; OVXe 0.14±0.01*, n=13; *p