CIC   05421
CENTRO DE INVESTIGACIONES CARDIOVASCULARES "DR. HORACIO EUGENIO CINGOLANI"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Effects of cannabidiol on infarct size and postischemic myocardial dysfunction; mechanisms involved
Autor/es:
FANTINELLI JC; MOSCA SM; GONZÁLEZ ARBELÁEZ LF; SEPÚLVEDA F
Lugar:
Buenos Aires
Reunión:
Congreso; Reunión de sociedades de biociencias 2020.LXV reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC), LXVIII reunión anual de la Sociedad Argentina de Inmunología (SAI), reunión anual de la Sociedad Argentina de Fisiología (SAFIS); 2020
Resumen:
EFFECTS OF CANNABIDIOL ON INFARCTSIZE AND POSTISCHEMIC MYOCARDIAL DYSFUNCTION: MECHANISMS INVOLVED Juliana CFantinelli 1, Luisa F González Arbeláez 1, Fernando JSepúlveda 2, Susana M Mosca 11 Centro deInvestigaciones Cardiovasculares ¨Dr Horacio E Cingolani¨, Facultad de CienciasMédicas, Universidad Nacional de La Plata, La Plata, ARGENTINA 2 Departamentode Bioquímica y Biología Molecular, Universidad de Concepción, Concepción, CHILE. Background: Cannabidiol (CBD) is a non psychoactivephytocannabinoid with recognized anti-inflammatory activity. Our aim was to determinethe effects of acute treatment of CBDon myocardial postichemic alterations and the mechanisms involved.Methods: Isolated Wistar rats hearts were isovolumicallyperfused through Langendorff system with Ringer´s solution (pH=7.4, 37°C) andpaced at 280 ± 10 beats/min. After 20 min of stabilization,the following experimental protocols were performed: Non-ischemic control(NIC):110 min of perfusion; Ischemic control (IC): 30 min of normothermic globalischemia and 60 min of reperfusion (R);CBD group: 0.25µM CBD was administeredduring the first 10 min of R. Infarct size (IS)was determined by TTC staining. Systolic function wasassessed by left ventricular developed pressure (LVDP) and +dP/dtmaxand diastolic function by left ventricular end diastolic pressure (LVEDP) and -dP/dtmax.The expression of phosphorylated forms of eNOS, PKCε, Akt and thecontent of cannabinoid receptor 2 (CB2) were determined by western blot. Results: CBD significantly decreased IS (7 ± 1 % vs. 31± 2 % in IC) and improved the post-ischemic recovery of myocardial function. At60 min of R, LVDP was 56 ± 8 % and +dP/dtmax  55 ± 8 % vs. 17 ± 3 %  and 15 ± 4 % in IC,respectively; LVEDP = 18 ± 3 mmHg vs. 52 ± 4 mmHg in IC; -dP/dtmax =58 ± 9 % vs. 14 ± 4 % in IC. The expression of P-eNOS andP-Akt decreased approximately 30% of NIC value (considered as 100 %) in IC and increasedapproximately 60% in CBD group. The expression of P-PKCε decreasedapproximately 50% in IC and increased a 40% in CBD group. The content of CB2 receptorsdiminished 30 % in IC hearts and increased 20 % in CBD treated hearts. Conclusions: The data demonstrate that CBD reduces the cell deathand systolic and diastolic post-ischemic dysfunction inducedby ischemia-reperfusion. These beneficial actions appear mediated by Akt/PKCe/eNOS-dependent pathways through CB2 receptors.