CIC   05421
CENTRO DE INVESTIGACIONES CARDIOVASCULARES "DR. HORACIO EUGENIO CINGOLANI"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Study of the biochemical and behavioral effects of omega-3 fatty acids on normotensive and hypertensive rats
Autor/es:
ZAVALA MAITE RAQUEL; FANANI MARÍA LAURA; MATÉ SABINA; FRANCO DOLCETTI; BELLINI MARÍA JOSÉ; LONGARZO MARÍA LUCRECIA; ROMINA VÁZQUEZ; MARÍA C. VILLA-ABRILLE
Lugar:
Santiago de Chile
Reunión:
Congreso; Asociación Latinoamericana de Ciencias Fisiológicas (ALACF); 2020
Institución organizadora:
Sociedad Chilena de Ciencias Fisiológicas-Asociación Latinoamericana de Ciencias Fisiológicas
Resumen:
IntroductionThe dietary intake of polyunsaturated fatty acids (PUFAs) of the omega-3 series -eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)- reduces the risk of cardiovascular diseases (CVD) and produces beneficial effects in patients with neurodegenerative diseases. The incorporation of omega-3 PUFAs into biological membranes changes membrane properties, and thereby affect signal transduction and cell function. ObjectivesAnalyze the effect of early supplementing in diet with EPA and DHA over depression symptoms and cardiac function in spontaneous hypertensive rats (SHR) compared with Wistar (W). Materials and MethodsTwenty one-day-old male Wistar and SHR rats (N=25) were randomly separated into four groups: Wistar, control and treated (W; W-T), and SHR, control and treated (SHR; SHR-T). The systolic pressure (SP) was measured and the Marble anxiety test was performed the day before the sacrifice. Blood, cardiomyocytes, and hippocampus were isolated and the total free fatty acids (FFA) composition was analyzed. Statistical analysis was performed by One-way ANOVA, followed by Tukey´s multiple comparisons test, p˂0,05. Handling and sacrifice of the animals were in full accordance with the Committee for the care and management of laboratory animals, UNLP (CICUAL).ResultsLower omega-6/omega-3 indexes were detected in the plasma of both W-T and SHR-T animals. Strikingly, the DHA percentage and DHA/Arachidonic Acid ratio were significantly higher in SHR than in W cardiomyocytes. No significant effect on the SP of SHR-T animals was detected. Similar levels of omega-3 PUFAs were found in the hippocampus of both W and SHR animals. Notwithstanding, a significant reduction in cardiac hypertrophy parameters and an increase in the digging activity of SHR-T animals were registered. ConclusionThese preliminary results show that omega-3 PUFAs intake could contribute to the prevention of the chronic inflammation associated with CVD and neurodegenerative diseases. However, the omega-3 PUFAs enrichment of biological membranes may not be the underlying mechanism responsible for these omega-3 FAs´ beneficial effects.