Transverse Aortic Constriction (TAC): Minimally Invasive Technique for induction of left ventricular hypertrophy in mice.

VELEZ RUEDA O; DIAZ ZEGARRA L; DE GIUSTI VC; ROSSETTI N; AIELLO EA

Congreso; Reunión anual de la Sociedad Argentina de Fisiología (SAFIS); 2019

Transverse aortic constriction (TAC) in mice is one of the most commonly used surgical techniques for experimental investigation of pressure overload-induced left ventricular hypertrophy induced by overload.In our laboratory we have developed a minimally invasive technique without intubation and ventilation of mice, significantly reducing surgical time and complications.Anesthesia: Ketamine and Xylazine. Cervicotomy. Dissection of pre tracheal muscles. Clips are placed in the Aortic arch, achieving an occlusion of 66% of the lumen.In conclusion, our cost-effective minimally invasive technique in mice has a very low operative and postoperative mortality close to 4% and is highly efficient in inducing LVH. Simplify the operating procedure and reduce the strain on the animal.