The activation of the G-Protein-Coupled Estrogen Receptor (GPER) decreases basal cardiac contractility

IBAÑEZ ALEJANDRO MARTÍN; ESPEJO MARÍA SOFÍA; DE GIUSTI VERÓNICA CELESTE; DIAZ ZEGARRA LEANDRO; AIELLO, ERNESTO ALEJANDRO

Bariloche

Congreso; The Fourth South American Symposium in Signal Transduction and Molecular Medicine; 2018

SISTAM

Over the past decade rapid effects of estrogens transduced by GPER have been discovered. Recently, this non-genomic signaling mechanism has been involved in cardioprotection through GPER activation by G1 (selective agonist). On the other hand, 17-beta-estradiol (E2) leads to rapid and transient activation of different intracellular signaling pathways like Ca2+ mobilization. In cardiac myocytes the Ca2+ management at the cytosol is critical for its contractile function. The project aims to reveal the role of GPER in basal cardiac contractility.Rat ventricular cardiac myocytes incubated with Fura2-AM dye were used to perform Ca2+ and contractile measurements in the Ionoptix System. Ca2+ influx through L-type calcium channel (ICaL) was assessed by the patch-clamp technique.The stimulation of GPER by G1 (1 μM) generated a negative inotropism in cardiac myocytes (% Sarcomere Length (SL), Control: 14.45±1.00, G1: 7.61±1.50, n=9, p