CIC   05421
CENTRO DE INVESTIGACIONES CARDIOVASCULARES "DR. HORACIO EUGENIO CINGOLANI"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
THE FUNCTIONAL ASSOCIATION BETWEEN THE SODIUM/BICARBONATE COTRANSPORTER AND THE SOLUBLE ADENYLATE CYCLASE (sAC) MODULATES BASAL CARDIAC CONTRACTILITY
Autor/es:
CIANCIO MC; DE GIUSTI VC; ORLOWSKI A; ESPEJO MS; AIELLO EA
Lugar:
S√łnderborg
Reunión:
Simposio; 57. Acta Physiologica International Symposium: H+ and HCO3- in Physiology and Pathophysiology. 49th Sandbjerg Meeting on membrane Transport.; 2017
Resumen:
The soluble AC (sAC) was identified in the heart as another source of cyclic AMP (cAMP). However, its cardiac physiological function is unknown. On the other hand, the cardiac Na+/HCO3- cotransporter (NBC) promotes the cellular co-influx of HCO3- and Na+. Since sAC activity is regulated by HCO3-, our purpose was to investigate the potential relationship between NBC and sAC. Rat ventricular myocytes were loaded with Fura-2 or Fluo-3 in order to measure Ca2+ transient amplitude (CaT) by epifluorescence or Ca2+ sparks frequency (CaSF) by confocal microscopy, respectively. Sarcomere shortening was measured simultaneously with epifluorescence. All the experiments were performed in a solution with bicarbonate. * indicates p
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