Compensatory role of the NBCn1 Na+/HCO3- co-transporter on Ca2+-induced mitochondrial swelling in spontaneously hypertensive rat heart mitochondria

VARGAS, LA.; ÁLVAREZ, BV; CARRIZO VELASQUEZ, F

Dublin

Congreso; Joint Meeting of the American Physiological Society and The Physiological Society; 2016

NBC Na+/HCO3- cotransporter and NHE1 Na+/H+ exchanger have been associated with cardiac disorders and recently located in mitochondria of cardiomyocytes and coronary endothelial cells (CEC), respectively. Mitochondrial NHE1 (mNHE1)blockade delay the mitochondrial permeability transition pore (MPTP)opening and reduce mitochondrial-derivedsuperoxide production, two critical events exacerbated in cells of the disease heart. Conversely, activation of the NBC isoform, NBCn1, prevented apoptosis in CEC subjected to ischemic stress. We characterize the role of these transporters in heart mitochondria of adult spontaneously hypertensive (SHR) and control (Wistar) rats. To examine the role of mNHE1 in mitochondria of SHR and Wistar rats, expression of mNHE1 in ventricular mitochondrial lysates was analyzed by immunoblots. mNHE1 expression increased by ~40% in hypertrophic SHR compared to control (P