Compensatory role of the NBCn1 Na+/HCO3- co-transporter on Ca2+-induced mitochondrial swelling in spontaneously hypertensive rat heart mitochondria

CARRIZO VELASQUEZ F; B.V. ÁLVAREZ; L.A. VARGAS

Dublin

Congreso; Joint Meeting of the American Physiological Society and The Physiological Society; 2016

NBC Na+/HCO3- cotransporter and NHE1 Na+/H+ exchanger have been associated with cardiac disorders and recently located in mitochondria of coronary endothelial cells (CEC) and cardiomyocytes, respectively. Mitochondrial NHE1 blockade delays mitochondrial permeability transition pore (MPTP) opening and reduces mitochondrial-derived superoxide production, two critical events exacerbated in cells of diseased hearts. Conversely, activation of the NBC isoform, NBCn1, prevented apoptosis in CEC subjected to ischemic stress. We characterized the role of these transporters in mitochondria of hearts from adult spontaneously hypertensive (SHR) and control (Wistar) rats. Expression of NHE1 was analyzed in left ventricular mitochondrial lysates, by immunoblots. NHE1 expression increased by ~40% in hypertrophic SHR compared to control (P