CENTRO DE INVESTIGACIONES CARDIOVASCULARES "DR. HORACIO EUGENIO CINGOLANI"
Unidad Ejecutora - UE
Aldosterone stimulates the cardiac sodium/bicarbonate cotransporter via activation of the g protein-coupled receptor gpr30
DE GIUSTI VERÓNICA C., ORLOWSKI ALEJANDRO; CIANCIO, MARÍA C.; ESPEJO MARÍA S.; GONANO, LUIS A.; CALDIZ, CLAUDIA I.; VILA PETROFF, MARTÍN G.; VILLA-ABRILLE MARÍA C.; AIELLO, ERNESTO A.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
ACADEMIC PRESS LTD-ELSEVIER SCIENCE LTD
Lugar: Amsterdam; Año: 2015 vol. 89 p. 260 - 267
Some cardiac non-genomic effects of aldosterone (Ald) are reported to be mediated through activation of the classic mineralocorticoid receptor (MR). However, in the last years, it was proposed that activation of the novel G protein-coupled receptor GPR30 mediates certain non-genomic effects of Ald. The aim of this study was to elucidate if the sodium/bicarbonate cotransporter (NBC) is stimulated by Ald and if the activation of GPR30 mediates this effect. NBC activity was evaluated in rat cardiomyocytes perfused with HCO3(-)/CO2 solution in the continuous presence of HOE642 (sodium/hydrogen exchanger blocker) during recovery from acidosis using intracellular fluorescence measurements. Ald enhanced NBC activity (% of ΔJHCO3(-); control: 100±5.82%, n=7 vs Ald: 151.88±11.02%, n=5; P