Effects of the Dietary Addition of Amaranth (Amaranthus mantegazzianus) Protein Isolate on Antioxidant Status, Lipid Profiles and Blood Pressure of Rats

LADO MB; BURINI J.; RINALDI G; AÑON MC; TIRONI V.

PLANT FOODS FOR HUMAN NUTRITION

SPRINGER

Lugar: Berlin; Año: 2015 vol. 70 p. 371 - 379

0921-9668

The effects of the dietary addition of 2.5 % (w/w)Amaranthus mantegazzianus protein isolate (AI) on bloodpressure, lipid profiles and antioxidative status of Wistar ratswere evaluated. Six diets were used to feed animals during28 days: (base (AIN93G), Chol (cholesterol 1 %, w/w), CE(α-tocopherol 0.005 %, w/w), CholE (cholesterol 1 % (w/w) +α-tocopherol 0.005 %, w/w), CAI (AI 2.5 % w/w), CholAI(cholesterol 1 % (w/w) + AI 2.5 %, w/w). Lipid profiles ofplasma and liver and faecal cholesterol content were analyzed.Antioxidant status was evaluated by the ferric reducing activityof plasma (FRAP), the 2-thiobarbituric acid (TBA) assayand superoxide dismutase (SOD) activity in plasma and liver.Blood pressure was measured in the tail artery of rats. CholAgroup presented a significant (α < 0.05) reduction (16 %) inthe plasma total cholesterol. In liver, the intake of cholesterol(Chol group) induced a significant increment in cholesteroland triglycerides (2.5 and 2.3 times, respectively), whichcould be decreased (18 % and 47 %, respectively) by theaddition of AI (CholA group). This last group also showedan increased faecal cholesterol excretion (20 %). Increment(50 %) in FRAP values, diminution of TBA value in plasmaand liver (70 % and 38 %, respectively) and diminution ofSOD activity (20 %) in plasma of CholA group suggest anantioxidant effect because of the intake of AI. In addition, CAand CholA groups presented a diminution (18 %) of bloodpressure after 28 days.