Protective effects of N-(2-mercaptopropionyl)-glycine against ischemia-reperfusion injury in hypertrophied hearts

FANTINELLI JC; GONZÁLEZ ARBELÁEZ LF; PÉREZ NÚÑEZ IA; MOSCA SM

EXPERIMENTAL AND MOLECULAR PATHOLOGY.

ACADEMIC PRESS INC ELSEVIER SCIENCE

Lugar: Amsterdam; Año: 2012

0014-4800

The beneficial effects of N-(2-mercaptopropionyl)-glycine (MPG) against ischemia-reperfusion injury in normotensive animals have been previously studied. Our objective was to test the action of MPG during ischemia and reperfusion in hearts from spontaneously hypertensive rats (SHR). Isolated hearts from SHR and age-matched normotensive rats Wistar Kyoto (WKY) were subjected to 50-min global ischemia (GI) and 2-hour reperfusion (R). In other hearts MPG 2 mM was administered during 10 min before GI and the first 10 min of R. Infarct size (IS) was assessed by TTC staining technique and expressed as percentage of risk area. Postischemic recovery of myocardial function was assessed. Reduced gluthathione (GSH), thiobarbituric acid reactive substances (TBARS) and SOD cytosolic activity- as estimators of oxidative stress and MnSOD cytosolic activity- as an index of (mPTP) opening were determined. In isolated mitochondria H2O2-induced mPTP opening was also measured. The treatment with MPG decreased infarct size, preserved GSH levels and decreased SOD and MnSOD cytosolic activity, TBARS concentration, and H2O2 induced-mPTP opening in both rats strains. Our results show that in both hypertrophied and normal hearts an attenuation of mPTP opening via reduction of oxidative stress appears be the predominant mechanism involved in the cardioprotection against reperfusion injury MPG-mediated.