CEPAVE   05420
CENTRO DE ESTUDIOS PARASITOLOGICOS Y DE VECTORES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
The Triatoma virus (TrV) : a structural approach by Cryo-Electron Microscopy
Autor/es:
EMMANUELLE NEUMANN; AGIRRE, J; GAËL GORET; MARTI G. A; NAVAZA J; GUERIN, D.M.A.
Lugar:
Lisboa
Reunión:
Workshop; ” I Iberoamerican Workshop on Chagas Disease, Triatomine vectors, T. cruzi, and Triatoma virus; 2010
Institución organizadora:
Centro de Malária e Outras Doenças Tropicais (UCDT/CMDT) Instituto de Higiene e Medicina Tropical
Resumen:
Capsid of a non-enveloped virus protects the genome from all forms of aggression in its passage from the cell in which it is produced to the cell where it will be produced. As a protective coat, the capsid must display a certain rigidity, but during uncoating it must become more flexible. Triatoma virus (TrV) is a viral pathogen of the blood-sucking reduviid bug Triatoma infestans, the main vector of the human Chagas disease. TrV virions consist of a non-enveloped capsid that encloses the viral genome, a single molecule of linear positive sense single-stranded RNA.             The purpose of this study was to determine the putative conformational changes which may occur when the capsid was empty of the genomic RNA and give new structural insight into the mechanisms of viral infection and capsid disassembly.             To this aim we used cryo-electron microscopy and 3D reconstruction techniques combined with X-ray crystallographic data.             We have shown that a cooperative movement of the three main viral proteins took place, leading to a channel which opened on the 5-fold axis which may allow the RNA to exit. These available data encourage us to continue efforts to discover the sequence of proteins movements during the uncoating process.