CEPAVE   05420
CENTRO DE ESTUDIOS PARASITOLOGICOS Y DE VECTORES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Shell disease by Vibrio sp. in grapsid crabs from Bahía Blanca estuary, Argentina
Autor/es:
MARTORELLI S.; ALDA P.; MARCOTEGUI P.; MONTES M.; PANEI C.J.
Lugar:
Buenos Aires
Reunión:
Congreso; 45th. Annual Meeting of the Society for Invertebrate Pathology- International Congress on Invertebrate Pathology; 2012
Institución organizadora:
Society for Invertebrate Pathology
Resumen:
In a survey carried out to increase the knowledge about pathogens of crustaceans in Argentinean wetlands, several species of shrimps and crabs were studied. In 5% of the grapsid crabs Neohelice granulata (n = 36) and Cyrtograpsus angulatus (n = 65) collected in Bahía Blanca estuary, erosive lesions of until 5 mm were observed in ventral and dorsal surfaces of shells. Some crabs with lesions were fixed in 10% formalin for histological studies. Others lesions were scraped in aseptic conditions and the material was spread in plates with TCBS and incubated at room temperature for 24􀍲48 hs. Positive plates showed round, yellow, and bright colonies integrated by Gram􀍲 negative bacteria. DNA extracted from this colonies was amplified by PCR using general primers for 16s rDNA segment and then sequenced. In histological sections of the cuticle very extensive foci were observed. Lesions were characterized for extended erosions throughout the epi􀍲, exo􀍲, and endocuticle, wich was often melanized. In the foci, bacteria, protozoans, and debris were observed. DNA sequences were compared to the ones deposited in GenBank (BLASTN) and the Ribosomal Database Project (RDP). We observed a close relationship with several Vibrio spp. Some of these species had been reported in association with crustacean culture: V. alginolyticus (96% BLASTN), V. furnissii (95% BLASTN), V. vulnificus (96% BLASTN), V. fluvialis (95% BLASTN), V. paccinii (85% RDP), and V. parahaemolyticus (86% RDP). Biochemical reactions will be necessary for the final identification of the causative agent of this shell disease.