CEPAVE   05420
CENTRO DE ESTUDIOS PARASITOLOGICOS Y DE VECTORES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Current State Of Knowledge About Oie-Notifiable Viral Pathogens In Crustaceans From Argentina
Autor/es:
MARTORELLI SERGIO R
Lugar:
Menphis
Reunión:
Conferencia; Aquaculture 2013; 2013
Institución organizadora:
World Aquaculture Society WAS
Resumen:
Since 2003, two of the most important commercially caught shrimp in Argentina (the Argentine stiletto shrimp, Artemesia longinaris, and the Pink shrimp Pleoticus muelleri) together with others crustaceans of ecological interest, were examined for parasites, epibionts, and pathogens. In 2008, several specimens of the penaeid shrimp A. longinaris from the BahBlanca estuary were found with numerous white spot in the cephalothorax shell (Fig.1). In some of these specimens, histological sections stained with H&E showed the presence of inclusion bodies Crowdy type A (Fig. 2), generally coincident with presence of White Spot Disease (WSD). In a first molecular study, using a commercial PCR kit, two specimens were found positive for the White Spot Syndrome Virus (WSSV). To confirm and double check the presence of WSSV in A. longinaris and in other two crustaceans, that were found also with spots in their cuticles (the grapsid crab Cyrtograpsus angulatus, and the introduced shrimp Palaemon macrodactylus), several specimens were analyzed in collaboration with Dr. Overstreet in the Gulf Coast Research Laboratory (USA). In this study three PCR methods and qPCR were used. In total, 56% of A. longinaris, 67% of C. angulatus, and 40% of P. macrodactylus were positive for WSSV. In addition, 30% of A. longinais was also positive for the Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV). In 2008- 2009, we found low copy numbers of the virus in most of the WSSV positive crustaceans and in 2010-2011 we did not found the virus. We suggest that low seawater temperatures in BahBlanca estuary during 2010-2011 may reduce the viral load (Fig.3). We are beginning to study the influence of water temperatures in the PCR detection of WSSV. Finally we analyzed three possible routes of introduction of the virus in Argentina. Our findings together with at least three other recent reports published from Brazil appear to confirm that WSSV is starting to establish in some populations of wild crustaceans from temperate waters of the South Atlantic Ocean influenced by the warm waters of the Brazil Current.