CEPAVE   05420
CENTRO DE ESTUDIOS PARASITOLOGICOS Y DE VECTORES
Unidad Ejecutora - UE
artículos
Título:
Cryo-electron microscopy reconstructions of triatoma virus particles: a clue to unravel genome delivery and capsid disassembly
Autor/es:
AGIRRE, J; GORET, G; LEGOFF, M; SANCHEZ- EUGENIA, R; MARTI, G. A; NAVAZA J; GUERIN, D.M.A.; NEUMANN, E
Revista:
JOURNAL OF GENERAL VIROLOGY
Editorial:
SOC GENERAL MICROBIOLOGY
Referencias:
Lugar: London; Año: 2013 vol. 94 p. 1058 - 1068
ISSN:
0022-1317
Resumen:
Triatoma virus (TrV) is a member of the insect virus
family Dicistroviridae and consists of a small, non-enveloped capsid that
encloses its positive-sense ssRNA genome. Using cryo-transmission electron
microscopy and three-dimensional reconstruction techniques combined with
fitting of the available crystallographic models, this study analysed the
capsids corresponding to mature and several RNA-empty TrV particles. After
genome release, the resulting reconstruction of the empty capsids displayed no
prominent conformational changes with respect to the full virion capsid. The
results showed that RNA delivery led to empty capsids with an apparent overall
intact protein shell and suggested that, in a subsequent step, empty capsids
disassemble into small symmetrical particles. Contrary to what is observed upon
genome release in mammalian picornaviruses, the empty TrV capsid maintained a protein shell thickness and size identical to
that in full virions.