CEPAVE 05420
CENTRO DE ESTUDIOS PARASITOLOGICOS Y DE VECTORES
Unidad Ejecutora - UE
artículos
Título:
Molecular techniques for Dicistrovirus detection without RNA extraction and purification.
Autor/es:
QUERIDO, J; AGIRRE, J; MARTI, G. A; GUERIN, D.M.A.; SOUSA SILVA, M
Revista:
JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY
Editorial:
HINDAWI PUBLISHING CORPORATION
Referencias:
Lugar: New York; Año: 2013 p. 1 - 3
ISSN:
1110-7243
Resumen:
Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines.The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus.
