CEPAVE 05420
CENTRO DE ESTUDIOS PARASITOLOGICOS Y DE VECTORES
Unidad Ejecutora - UE
artículos
Título:
Entomopathogenic fungi against Schistocerca cancellata (Orthoptera: Acrididae): relationship between fungal pathogenecity and proteolytic, lipolytic and chitinolytic activity.
Autor/es:
PELIZZA, S.; ELÍADES, L.A.; SCORSETTI, A.C.; CABELLO, M.N.; LANGE, C.E.
Revista:
BIOCONTROL SCIENCE AND TECHNOLOGY
Editorial:
TAYLOR & FRANCIS LTD
Referencias:
Lugar: Londres; Año: 2012 vol. 22 p. 1119 - 1129
ISSN:
0958-3157
Resumen:
The South American locust Schistocerca cancellata (Serville) was the most serious agricultural pest in Argentina during the first half of the last century and remains as a threat when preventive control measures are relaxed in the outbreak area. Susceptibility of S. cancellata to pathogens such as Malameba locustae, Melanoplus sanguinipes Entomopox virus (MsEPV), and Paranosema locustae has been demonstrated under laboratory conditions, but the effects caused by entomopathogenic fungi have never been studied. At present, chemical insecticides are still the only means utilized for control of S. cancellata, but their use is of significant environmental concern. Since the insect cuticle is the first barrier encountered by fungi, they synthesize a wide variety of extracellular enzymes involved in the degradation of protein, chitin and lipids, which are the principal components of the cuticle. In this study, we analyzed in the laboratory the effectiveness of 26 fungal strains (isolated from both insects and soil collected in Argentina) for S. cancellata control and determined the relationship between the chitinase, protease, and lipase levels in these fungi and their insecticidal activities. We observed that Beauveria bassiana (isolate LPSC 1067) caused the highest mortality (90 + 1.03 %), the highest values of chitinolytic, proteolytic and lipolytic activity were 6.13 + 0.05; 2.56 + 0.11, and 2.33 + 0.47, respectively and a the lowest median lethal time was 5.96 days. This is the first time that a wide variability in chitinase, protease and lipase activity as well as in virulence has been reported in a representative sample of different entomopathogenic fungal strains from Argentina.
