CEPAVE   05420
CENTRO DE ESTUDIOS PARASITOLOGICOS Y DE VECTORES
Unidad Ejecutora - UE
artículos
Título:
Characterization and phylogeny of entomopathogenic Isaria spp. (Ascomycota: Hypocreales) using ITS1-5.8S-ITS2 and elongation factor 1-alpha sequences.
Autor/es:
D'ALESSANDRO C.P; JONES L.; HUMBER R.; LOPEZ LASTRA C.C; SOSA-GOMEZ D.R
Revista:
FEMS MICROBIOLOGY LETTERS
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Año: 2011
ISSN:
0378-1097
Resumen:
The elongation factor 1-alpha (EF1-á) and the internal transcribed spacer regions ITS1 and ITS2 (ITS1-5.8S-ITS2) sequences were used to characterize and to identify Isaria isolates from Argentina and Brazil, as well as to study the phylogenetic relationships among these isolates and other related fungi from the order Hypocreales. The molecular characterization, which was performed by PCR-RFLP of EF1-á and ITS1-5.8-ITS2 genes, was useful for resolving the representative isolates of Isaria fumosorosea, Isaria farinosa and Isaria tenuipes and to confirm the taxonomic identity of fungi from Argentina and Brazil. The phylogenetic analyses showed three clades corresponding to three families of Hypocreales. The genus Isaria was redefined as monophyletic and in Cordycipitaceae. Isaria species were related to anamorphic Beauveria, Lecanicillium and Simplicillium genera and to teleomorphic Cordyceps and Torrubiella genera. Therefore, EF1-á and ITS1-5.8S-ITS2 genes were found to be powerful tools for improving the characterization, identification and phylogenetic relationship of the Isaria species and other entomopathogenic fungi.