LACK OF ENDOGENOUS ESTRADIOL DID NOT WORSEN GENERAL METABOLIC STATE NOR ADIPOSE TISSUE FUNCTION, EVEN THOUGH ADVERSE DIET CONSUMPTION

MARTIN F; SPINEDI E; ALZAMENDI A; GIOVAMBATTISTA A; MIGUEL I; REY A

Mar del Plata

Congreso; LXIV Reunión Anual Sociedad Argentina de Investigación Clínica; 2019

Sociedad Argentina de Investigación Clínica

We previously studied the role of estrogen deficiency in 60-day-old female rats ovariectomized (OVX) on prepubertal age (27 day-old). It was concluded that OVX animals have improved some metabolic-endocrine functions. Our aim was to determine consequences of fructose rich diet (FRD) intake on female rats OVX at adult age. Therefore, OVX rats were fed FRD (fructose 10% w/v in tap water) between 60-81 days of age. Thus groups studied were: animals that consumed water and chow ad libitum: control basal (CTR B), ovariectomized basal (OVX B), and considering higher food intake by OVX animals, OVX pair-fed rats (OVX PF B) were included; finally, rats in similar conditions but fed FRD (CTR FRD, OVX FRD, OVX PF FRD) were included. Trunk blood was collected to measure plasma metabolites and retroperitoneal adipose tissue (RPAT) pads were dissected out, weighed and processed for histological and gene expression analyses. FRD treatment did not increased body weight in any rat, despite energy intake. RPAT mass decreased in OVX PF B vs CTR B animals (p