In vivo and in vitro dexamethasone treatment enhances white adipocyte precursor cells competency.

SPINEDI E; ZUBIRÍA, MARÍA GUILLERMINA; GIOVAMBATTISTA, ANDRÉS; GIORDANO, A

Mar del Plata

Congreso; LXIV REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC); 2019

SAIC

Dexamethasone (DXM) is a synthetic glucocorticoid widely recognized as an adipogenic inducer in both, in vivo and in vitro models. However, there is scarce information about the DXM role in adipocyte precursor cells (APC) before differentiation induction. Our aim was to evaluate the DXM effect on APC number and competency, using in vivo and in vitro approaches. Therefore, we injected two groups of adult male rats for 2 or 7 days with either DXM (30 g/kg of weight ip, DXM-d2 and DXM-7d, respectively) or vehicle (CTR-2d and CTR-7d). Retroperitoneal adipose tissue (RPAT) was dissected and stromal vascular fraction (SVF) cells were isolated for Fluorescence-Activated Cell Sorting (FACS) analysis (APC profile: CD34+/CD45-/CD31-) and expression of competency markers (PPAR2 and Zfp423). Two days after DXM treatment PPAR2 and Zfp423 expression levels were increased (P