EFFECT OF MATERNAL FRUCTOSE-RICH DIET (FRD) INTAKE ON THE ADULT OFFSPRING BROWNING POTENTIAL OF RETROPERITONEAL WHITE ADIPOSE TISSUE (RPWAT).

ALZAMENDI A; GIOVAMBATTISTA A; MIGUEL I; SPINEDI E

Mar del Plata

Congreso; LXIV Reunión Anual Sociedad Argentina de Investigación Clínica; 2019

Sociedad Argentina de Investigación Clínica

Beige adipocytes dissipate energy as heat through uncoupling protein-1 (UCP1) activity. Cold exposure or β3-adrenergic agonist treatment stimulates white to beige adipocyte conversion. Although not completely matched, UCP1 expression levels are largely related to beige adipocyte thermogenesis. Our aim was to assess whether maternal FRD intake throughout pregnancy affects the development of browning capacity in RPWAT from the adult male offspring. On pregnancy day 1, dams were provided with either tap water (control) or FRD (10%w/v; in tap water) and fed ad libitum with chow up to delivery. Weaned pups received water and chow ad libitum up to 60 days of age (experimental day). C and F indicate pups born to control and FRD dams, respectively. On day 53 of age, C and F pups were submitted to cold (4ºC, for one week). Body weight and food intake was daily registered. On experimental day, trunk blood was collected and RPWAT pads were dissected and weighted. RPWAT mRNA expression levels of beige adipogenic markers were measured, and additional tissue was used for H&E staining, IHC and western blot analysis. Data indicate that basal Pgc1α and UCP1 expression were lower in F rats (p