Effect of the Lack of Post-Pubertal, Ovary-Dependent Programming on Metabolic-Endocrine and Liver Functions

VILLAGARCÍA H; SPINEDI E; CASTRO MC; FRANCINI F; MASSA ML

Orlando

Congreso; ENDO2017 The Endocrine Society´s 99th annual meeting; 2017

Background & Aim: Post-pubertal bilateral ovariectomy (OVX)-induced Metabolic Syndrome (MS) phenotype in rats is characterized by increased body weight (BW)/fat and endocrine-metabolic dysfunctions. Our aim was to evaluate the effect of the lack of pubertal programming, by the endogenous peak of ovary-derived estradiol (E2), on endocrine-metabolic and liver functions when adulthood (90 days old) was reached. Materials and Methods: Sprague-Dawley rats were either OVX on day 23/25 of life (pre-pubertal OVX; ppOVX) or sham (ppSHX) operated (dorsal approach) under mild anesthesia. Recovered rats were allocated 3 per cage, with free access to rat chow and water up to 69 days of age; then divided in groups either receiving drinking water (C) or fructose rich diet (FRD; 10% F in drinking water and rat chow provided ad libitum during 3 weeks). Built groups were: ppSHX-C, ppOVX-C, ppSHX-F and ppOVX-F. Subsets of these groups were sacrificed in non-fasting (basal) condition or, after overnight fasting, submitted to an ip glucose tolerance test (GTT; 2 g/kg BW). BWs, food-intake and drank solution were recorded day after day. Circulating levels of glucose (GLU), triglyceride (TG) and estradiol (E2) were quantified. Glycemias were monitored throughout (0-120 min) the GTT, and individual area under the curve (AUC) of circulating GLU levels was calculated. Finally, liver gene expression levels of lipogenesis-related proteins (SREBP-1c, FAS and GPAT) and estrogen receptor-A (ER-A) were quantified. Results: Non-fasting ppOVX rats showed (p