Angiogenesis and VEGF-A pathway are involved in the mechanism by which INGAP_PP increases B-cell mass and function

MAIZTEGUI B; GAGLIARDINO JJ; ROMAN CL; DEL ZOTTO H; FLORES LE

Buenos Aires

Congreso; Pancreas 2017 - 21st IAP-LAPSG Joint Meeting; 2017

Background:Islet angiogenesis is needed for embryonic β-cell differentiation and INGAP-PP administration increases Beta-cell mass and function in rats.Aim:To determine the role of islet angiogenesis in the mechanism by which INGAP-PP enhances β-cell mass and function. Methods: In vivo: adult Wistar rats were treated (ip) for 10 days with saline (Control) or INGAP-PP (500µg/day). Thereafter, serum parameters levels were measured and HOMA-IR and ? β were calculated. Cell apoptotic rate and gene expression (qPCR and western blot) of angiogenesis and apoptotic markers were determined. In vitro: islets isolated from normal rats were cultured for 4 days in different composition: Control; INGAP-PP (10µg/ml); Rapamycin (10ng/ml + control or INGAP-PP); and VEGF (10ng/ml). We determined VEGF released to the medium (ELISA) and glucose-stimulated insulin secretion (RIA) Statistics: ANOVA and t test; significant differences were considered when p