CENEXA   05419
CENTRO DE ENDOCRINOLOGIA EXPERIMENTAL Y APLICADA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Testosterone effects on the thermogenic program of the inguinal adipose tissue from the adult rat.
Autor/es:
HARNICHAR AE; GIOVAMBATTISTA A; SPINEDI E; ZUBIRIA G
Lugar:
CABA
Reunión:
Congreso; LXII Reunion de la Sociedad Argentina de Investigacion Clinica (SAIC); 2017
Institución organizadora:
SAIC
Resumen:
Little is known regarding the effects of androgens on the thermogenic process of beige adipocytes. We previously demonstrated an inhibitory activity of Testosterone (T), in vivo and in vitro, on the thermogenic process of retroperitoneal adipose tissue (AT) cells. In the present study we evaluated the effect of T on the thermogenic activity of the subcutaneous AT (Inguinal AT: IAT). For this aim, three groups of male S-D rats were build: control (CTR), pre-pubertally (27 day-old) orchidectomized (ODX) and control pair-fed (CTR-PF). At age 60 days, the IAT pad was dissected and adipocyte precursor cells (APCs) were isolated. APCs were cultured and differentiated (3 days) with a classic pro-beige cocktail. On differentiation day (Dd) 8, cells were processed to quantify UCP-1 gene expression (qPCR). Additionally, IAT APCs from adult CTR rats were isolated and differentiated with the classic pro-beige cocktail in the absence or presence of 0.1 µM T (basal: B or T, respectively), thereafter cells remained in culture without or with T. On Dd 8, 10 µM FSK was added for 4 h to subsets of B and T cells: B without FSK (B-B), B with FSK (B-FSK), T without FSK (T-B) and T with FSK (T-FSK). Cells were then processed to quantify UCP-1 mRNA levels. APCs from ODX animals differentiated in vitro showed a higher UCP-1 mRNA expression (p