Maternal fructose rich diet intake throughout gestation inhibits the adipogenic potential of white adipose tissue precursor cells from the male offspring.

ALZAMENDI A; SPINEDI E; PORTALES A; ZUBIRIA G; GIOVAMBATTISTA A

Mar del Plata

Congreso; LXI Reunion de la Sociedad Argentina de Investigacion Clinica (SAIC).; 2016

Previous studies from our groupindicate that fructose rich diet (FRD) consumption during gestationinduces a decrease in adipose precursor cells (APCs) from retroperitonealadipose tissue (RPAT) thus reducing local adipocyte number and mass in theadult male progeny. These changes favor adipocyte hypertrophy and adistorted adipokine secretion pattern. We now evaluated the impact of FRDintake by the gestating dams on the adipogenic capacity of stromal vascularfraction (SVF) cells from RPAT, of their adult male progeny. On pregnancy day1, dams were provided with either tap water alone (control) or containingfructose (10% w/v in drinking water; FRD) and fed ad libitum with chow up to delivery.Lactating dams and their pups (between 21 and 60 of age) received water andchow ad libitum. C and F animals will comefrom control and FRD dams, respectively. On experimental day (age 60 days) RPATpads were dissected and SVF cells were isolated. The mRNA expression levels ofadipogenic potentialmarkers were assessed by qPCR in RPAT SVF cells and pads. PPARγ expression was quantified in differentiating cells and markers of terminaldifferentiation were also evaluated. Our data indicate that local F SVF cellsexpress lower and higher levels (p<0.05) of CD34 and Pref-1,respectively. Immunofluorescence for PPARγ labeledcells (on day 4 post-differentiation) was lower in F than C animals(p<0.05). On day 10 post-differentiation, a lower number ofdifferentiating F cells was noticed (p<0.05 vs C). Increased Ob and LPL geneexpression was found in RPAT pads from F rats (p<0.05). These resultsindicate that pre-natal nutritional intervention induces in adult male progenya decrease in the adipogenic potential of RPAT APCs, favoring an unhealthypad mass expansion (hypertrophic). A dysfunctional RPAT strongly contributes todevelop multiple endocrine-metabolic disorders. PICT-2013-0930/CICPBA/FPREDM052015.