CENEXA   05419
CENTRO DE ENDOCRINOLOGIA EXPERIMENTAL Y APLICADA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Endogenous Sex Steroid-Dependent in Vitro Rat Adipogenesis.
Autor/es:
MARTÍN MF; FARIÑA JP; CASTROGIOVANNI D; GIOVAMBATTISTA A; GAGLIARDINO JJ; SPINEDI E
Lugar:
Chicago
Reunión:
Congreso; ICE/ENDO 2014; 2014
Institución organizadora:
ESO
Resumen:
Aim: To assess whether the endogenous sex steroid milieu could be regulating the in vitro adipogenic capacity of stromal-vascular fraction cells (SVFs) isolated from rat white adipose tissue (AT) pad paracrinally related to the reproductive axis function (epididymal and parametrial: EAT and PAT). Design & Measurements: Twenty five-old Wistar male and female rats were either bilaterally gonadectomized (ODX, OVX) or sham operated (M, F) and left undisturbed until experimentation (60-70 days of age). After sacrificed in non fasting condition, trunk blood was collected (testosterone and estradiol were measured) and, EAT/PAT pads were aseptically dissected, weighed, and processed for SVF cells isolation. After counted, a similar number of SVFs was seed and cultured up (6-7 days) to 60-70 % confluence and counted (proliferation). Thereafter, cells were induced to differentiate in a 10 day-culture schedule. At the end, cells were stained with Oil Red O (ORO) and color/DNA were quantified (490/260 nm). Results: Pre-pubertal gonadectomy significantly (P < 0.05) reduced the homologous sex steroid peripheral level. One mg of AT rendered more (P < 0.05) SVFs in F-PAT than in M-EAT pads, and this count was significantly (P < 0.05 vs. F-PAT) reduced in OVX-PAT only. We recorded only a tend to enhanced proliferative capacity in SVFs, expressed in a gender-dependent fashion (F=OVX>M=ODX; P = 0.049). Finally, the adipogenic capacity in SVFs displayed a graded order: F=OVX=ODX>M; P < 0.05). Conclusions: Our data clearly indicate that gender-dependent differences in the distribution and expansion processes of the white AT are clearly related to the endogenous sex steroid-rich milieu.