CENEXA   05419
CENTRO DE ENDOCRINOLOGIA EXPERIMENTAL Y APLICADA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Increased in vitro adipogenesis in the juvenile hypothalamic obese male rat.
Autor/es:
ZUBIRIA G; SPINEDI E; GIOVAMBATTISTA A
Lugar:
Quebec
Reunión:
Congreso; 3rd. Congress on Abdominal Obesity; 2012
Institución organizadora:
Int. Society on Abdominal Obesity
Resumen:
Objective: To evaluate the in vitro adipogenic capacity of stromal vascular fraction (SVF) cells from 30 day-old normal (CTR) and neonatally Lmonosodium glutamate (MSG) treated male rats. Methods: At experimental day peripheral blood was collected and the levels of leptin (LEP), insulin (INS) and corticosterone (B) were determined. Retroperitoneal adipose tissue (RPAT) pad was dissected and weighted, and cells from the SVF were isolated. SVF cells in culture were allowed to proliferate up to reach confluence and pre-adipocyte differentiation was induced (day 0), then cells were cultured up to day 10 post-differentiation. Between days 0 and 10 of the differentiation period, media LEP concentrations were monitored. Intracellular lipid content (Oil-Red O) and cell gene expression (LEP, adiponectin and PPAR-γ mRNA levels) were also examined at the end of the differentiation period (day 10). Results: MSG rats displayed high plasma LEP levels and RPAT mass, whereas peripheral INS and B levels remained normal. On day 10 post-differentiation, MSG cells showed an increased (p < 0.05 vs. CTR) LEP release into the medium and lipid content. Finally, mature MSG adipocytes displayed higher LEP (p < 0.05 vs. CTR) and PPAR-γ (albeit not significant) mRNA levels, although similar adiponectin gene abundance. Conclusions: Our study indicates that in the juvenile MSG male rat, the in vitro adipogenic capacity seems to be enhanced. It is suggested that the increased RPAT mass found in MSG animals could be due, at least in part, to hyperplasic expansion before the development of an endogenous glucocorticoid-rich milieu.(PICT 2007-1051)