Long-Term Fructose Intake Increases Adipogenic Potential: Evidence of Direct Effects of Fructose on Adipocyte Precursor Cells

ZUBIRIA G; ALZAMENDI A; MORENO G; REY MA; SPINEDI E; GIOVAMBATTISTA A

Nutrients

Hindawi Publishing Corporation

Año: 2016 vol. 8 p. 1981 - 19815

We have previously addressed that fructose rich diet(FRD) intake for three weeks increases the adipogenic potential of stromalvascular fraction cells from the retroperitoneal adipose tissue (RPAT). We havenow evaluated the effect of prolonged FRD intake (eight weeks) on metabolicparameters, number of adipocyte precursor cells (APCs) and in vitro adipogenicpotential from control (CTR) and FRD adult male rats. Additionally, we haveexamined the direct fructose effects on the adipogenic capacity of normal APCs.FRD fed rats had increased plasma levels of insulin, triglyceride and leptin,and RPAT mass and adipocyte size. FACS studies showed higher APCs number andadipogenic potential in FRD RPAT pads; data is supported by high mRNA levels ofcompetency markers: PPARγ2 and Zfp423.Complementary in vitro experimentsindicate that fructose-exposed normal APCs displayed an overall increasedadipogenic capacity. We conclude that the RPAT mass expansion observed in eightweek-FRD fed rats depends on combined accelerated adipogenesis and adipocytehypertrophy, partially due to a direct effect of fructose on APCs.