CENEXA   05419
CENTRO DE ENDOCRINOLOGIA EXPERIMENTAL Y APLICADA
Unidad Ejecutora - UE
artículos
Título:
Apocynin administration prevents the changes induced by a fructose-rich diet on rat liver metabolism and the antioxidant system
Autor/es:
CASTRO MC; FRANCINI F; SCHINELLA G; CALDIZ C; ZUBIRIA M; GAGLIARDINO JJ; MASSA ML
Revista:
CLINICAL SCIENCE (LONDON, ENGLAND : 1979)
Editorial:
PORTLAND PRESS LTD
Referencias:
Lugar: Londres; Año: 2012 vol. 123 p. 681 - 692
ISSN:
0143-5221
Resumen:
The aim of this study was to evaluate the role of NAD(P)H oxidase in liver oxidative stress (OS), the metabolic changes induced by a fructose-rich diet (FRD), and their prevention by apocynin co-administration. Wistar rats were fed for 21 days a) a control diet, b) a control diet plus 10% fructose in the drinking water, and c) a control and FRD diet plus apocynin in the drinking water. Glycemia, triglyceridemia and insulinemia were measured and the homeostasis model of assessment of insulin resistance (HOMA-IR) ratio calculated. In liver we measured a) NAD(P)H oxidase activity, gene and protein expression, b) protein carbonyl groups, reduced glutathione (GSH) and lipid peroxidation, c) catalase, CuZn-superoxide dismutase (CuZnSOD) and Mn-superoxide dismutase (MnSOD) gene and protein expression, d) liver glycogen and triglyceride content, and e) glucokinase (GK), glucose-6-phosphatase (G-6-Pase) and glucose-6-phosphate dehydrogenase (G-6-P DH) activities. FRD animals showed hypertriglyceridemia, hyperinsulinemia and higher activity/expression of NAD(P)H oxidase, increased OS markers, reduced antioxidant enzyme expression, enhanced liver glycogen and triglyceride storage, and increased GK, G-6-Pase and G-6-P DH activities. In FRD rats, apocynin co-administration reduced triglyceridemia but not hyperinsulinemia, and normalized NAD(P)H oxidase expression and activity together with OS markers and antioxidant enzymes. Apocynin also prevented hepatic triglyceride accumulation. In conclusion, NAD(P)H oxidase plays a key role in FRD-induced hepatic reactive oxygen species production and could also be involved in liver steatosis. Apocynin prevents most of these alterations, thus suggesting its potential usefulness for the prevention/treatment of the metabolic syndrome and type 2 diabetes mellitus.