INFIVE   05416
INSTITUTO DE FISIOLOGIA VEGETAL
Unidad Ejecutora - UE
artículos
Título:
Tomato SlWak1 depends on Fls2/Fls3 to promote apoplastic immune responses to Pseudomonas syringae
Autor/es:
ROSLI, HERNÁN GUILLERMO; NING ZHANG; MARTIN, GREGORY; POMBO, MARINA ALEJANDRA
Revista:
PLANT PHYSIOLOGY.
Editorial:
AMER SOC PLANT BIOLOGISTS
Referencias:
Lugar: Rockville; Año: 2020
ISSN:
0032-0889
Resumen:
Wall-associated kinases (Waks) are known to be important components of plant immunity against various pathogens, including Pseudomonas syringae pv. tomato (Pst) although their molecular mechanisms are largely unknown. In tomato, SlWak1 has been implicated in pattern recognition receptor-(PRR)-triggered immunity (PTI) because its transcript abundance increases significantly after treatment with the flagellin-derived microbe-associated molecular patterns (MAMPs) flg22 and flgII-28, which activate the PRRs Fls2 and Fls3, respectively. We generated two SlWak1 tomato mutants (Δwak1) using CRISPR/Cas9 and investigated the role of SlWak1 in tomato-Pst interactions. Late PTI responses activated in the apoplast by flg22 or flgII-28 were compromised in Δwak1 plants but PTI at the leaf surface was unaffected. The Δwak1 plants developed fewer callose deposits than wild-type plants but retained early PTI responses such as generation of reactive oxygen species and activation of MAPKs upon exposure to flg22 and flgII-28. The induction of Wak1 gene expression by flg22 and flgII-28 was greatly reduced in a tomato mutant lacking Fls2 and Fls3 but induction of Fls3 gene expression by flgII-28 was unaffected in Δwak1 plants. After Pst inoculation, Δwak1 plants developed disease symptoms more slowly than Δfls2.1/2.2/3 mutant plants, although both plants ultimately were similarly susceptible. SlWak1 co-immunoprecipitated with both Fls2 and Fls3 independently of flg22/flgII-28 or BAK1. These observations suggest that SlWak1 acts in a complex with Fls2/Fls3 and plays an important role at later stages of PTI in the apoplast.