Characterization of the primary metabolome during the long-term response to NaHCO3-derived alkalinity in Lotus japonicus ecotypes Gifu B-129 and Miyakojima MG-20

RUBEN ROCCO; FRANCISCO J. ESCARAY; PEDRO CARRASCO; MARIA FLORENCIA BABUIN; ANDRES GARRIZ; OSCAR A. RUIZ; CESAR D. BORDENAVE; MARIA PAULA CAMPESTRE; CRISTIAN ANTONELLI; ANA B. MENENDEZ

ACTA PHYSIOLOGIAE PLANTARUM

SPRINGER HEIDELBERG

Lugar: HEIDELBERG; Año: 2019 vol. 39 p. 76 - 82

0137-5881

The present study was aimed at analyzing alkalinity-induced changes in the global metabolome of the L. japonicus ecotypes Gifu B-129 and Miyakojima MG-20. GC-MS analysis of leaf extracts from both ecotypes yielded 41 different, quantificable metabolites, including organic acids (OA), amino acids (AA), sugars and polyols. Alkalinity did not affected the total amount of OA, but increased those of AA in both ecotypes and glucids in MG-20, while it reduced the overall amount of polyols in MG-20. Some metabolites were present or changed similarly in both ecotypes, whereas others did it in a genotype-specific maner. Among the first group were citramalic and citric acids, asparagine, D-xylulose, ononitol and its precursor myo-inositol. Among the genotype-specific metabolites afected by alkalinity were lactic and phosphoric acids valine, β-alanine, tyramine, serine and proline. Our results showing serine accumulation and reduction in available Fe observed in alkalinized Gifu B-129 plants put forward the hypothesis that alkalinity hindered the photorespiration activity in these plants, whereas variations in xylose and p-Coumaric acid accumulations between both ecotypes suggests the notion that alkalinity negatively affected the cell wall strength of Gifu B-129, but not that of MG-20 plants