INFIVE   05416
INSTITUTO DE FISIOLOGIA VEGETAL
Unidad Ejecutora - UE
artículos
Título:
Enhancing laccase production by white-rot fungus Funalia floccosa LPSC 232 in co-culture with Penicillium commune GHAIE86
Autor/es:
SILES, JOSÉ A.; GARCÍA-ROMERA, INMACULADA; RODRÍGUEZ, ROSARIO DÍAZ; JURADO, MIGUEL; SAMPEDRO, INMACULADA; HEREDIA, GABRIELA; SAPARRAT, MARIO CARLOS NAZARENO
Revista:
FOLIA MICROBIOLOGICA
Editorial:
SPRINGER
Referencias:
Año: 2019 vol. 64 p. 91 - 99
ISSN:
0015-5632
Resumen:
To obtain enzymatic preparations with higher laccase activity levels from Funalia floccosa LPSC 232, available for use in several applications, co-cultures with six filamentous microfungi were tested. A laccase non-producing soil fungus, identified as Penicillium commune GHAIE86, showed an outstanding ability to increase laccase activity (3-fold as compared to that for monoculture) when inoculated in 6-day-old F. floccosa cultures. Maximum laccase production with the F. floccosa and P. commune co-culture reached 60 U/mL, or twice that induced by chemical treatments alone. Our study demonstrated that co-culture with soil fungi might be a promising method for improving laccase production in F. floccosa. Although the enhancement of laccase activity was a function of P. commune inoculation time, two laccase isoenzymes produced by F. floccosa remained unchanged when strains were co-cultured. These data are compatible with the potential of F. floccosa in agricultural applications in soil, whose enzyme machinery could be activated by soil fungi such as P. commune.