Nitric oxide in combination with indole-3-butyric acid improves root growth in `Ferdor Julior´ hardwood cuttings (Prunus insistitia (L.) x Prunus domestica (L.))

GERGOFF GROZEFF, G.E.; ROMERO, MARÍA DE LOS ÁNGELES; AUBONE VIDELA, M.

Journal of Horticultural Science and Biotechnology

Taylor and Francis

Año: 2017 vol. 93 p. 175 - 184

1462-0316

The production of plants for high density fruit orchards requires a highly efficient clonal propagation method. `Ferdor Julior´ rootstock is well adapted to different soil conditions, but its natural rooting potential is very limited. Propagation treatments such as bottom heat and indole-3-butyric acid (IBA) applications have been previously used to improve rooting. The aim of this work is to evaluate the effect of: either IBA, or sodium nitroprusside (SNP) (a nitric oxide (NO) donor) or a combination of both on the rooting of hardwood cuttings of `Ferdor Julior´. As metabolism markers, ascorbic acid (AA) and glutathione (GSH) were measured in adventitious roots.`Ferdor Julior´ hardwood cuttings were treated as follows: untreated, 0.3 mM IBA, 1 mM SNP, 1 mM SNPi (as a negative control), 1 mM SNP + 0.3 mM IBA. After 4 months the combined use of SNP and IBA increased root growth and lateral rooting but resulted in less shoot growth; while AA and GSH concentration were also reduced. IBA and SNP individual treatments showed intermediate results compared to untreated cuttings. According to the results obtained, there may be an additive effect over the auxin and the NO signaling pathways. In conclusion, this new promising technique for ?Ferdor Julior? nursery could improve lateral root development and promote early lateral root growth.