INSTITUTO DE FISIOLOGIA VEGETAL
Unidad Ejecutora - UE
New media for increasing sporulation and germination of Phaeoisariopsis griseola conidia
STENGLEIN S.A., AULICINO M., ARAMBARRI A.M. & BALATTI P.A.
EUROPEAN JOURNAL OF PLANT PATHOLOGY
Año: 2006 vol. 115 p. 173 - 173
The slow growth rate of the fungus Phaeoisariopsis griseola and the availability of a homogeneous highly concentrated inoculum is an important constraint for pathogenicity or virulence studies, where plant inoculations are needed. Therefore, the purpose of this paper was to evaluate the effect, supplementation of culture media with Amaranthus cruentus seed meal, has on fungal growth and sporulation of isolates of P. griseola belonging to the Mesoamerican and Andean group. The amendment of PDA or V8 media with A. cruentus seed meal resulted in a considerable increase in the number of conidia and also in their capacity to germinate, which seems to depend mostly on the stage of maturity of conidia. It seems that either one or several of the nutritional factors provided by A. cruentus contributed to accelerate the production of conidia and their development, resulting in faster development of the disease and an earlier appearance of symptoms. Therefore, for cultural studies, especially for inoculum production and for pathogenicity evaluations, supplementation of the media with A. cruentus seed meal proved to be a good alternative.