INFIVE   05416
INSTITUTO DE FISIOLOGIA VEGETAL
Unidad Ejecutora - UE
artículos
Título:
Growth and oxidative enzimatic activity of in- vitro culture of Ciliochorella buxifolia
Autor/es:
MARIA INÉS TRONCOZO; ROMINA P GÓMEZ; ARAMBARRI A. M.; BALATTI PEDRO A.; BUCSINSZKY, A. M. M.; MARIO CARLOS NAZARENO SAPARRAT
Revista:
MYCOSCIENCE
Editorial:
SPRINGER TOKYO
Referencias:
Lugar: Tokyo; Año: 2015 vol. 56 p. 58 - 58
ISSN:
1340-3540
Resumen:
To get a better insight into the physiological capabilities of Ciliochorella buxifolia, the most frequent fungus occurring on Scutia buxifolia leaf-litter in a native forest from Argentina, its in-vitro ability to use 10 carbon sources and to produce extracellular enzymes, including its response to tannic acid and to the addition of a water-soluble fraction of Scutia buxifolia leaf-litter, was analyzed. Growth, colony morphology and extracellular enzyme activity as well as differentiation of pycnidia were a function of the C substrate. The fungus responded to the presence of tannic acid in a range between 0.001 and 0.1% (w/v), by increasing growth, but higher phenol concentrations like 0.5% were inhibitory. The activity of extracellular oxidative enzymes increased with the concentration of tannic acid. Furthermore, the fungus showed extracellular laccase and peroxidase activity, being the former increased by water-soluble fraction in association to pycnidia development. Based on these results, Ciliochorella buxifolia is a fungus growing on Scutia buxifolia leaf-litter that is able to metabolize soluble phenolic compounds, which triggers the synthesis of extracellular oxidative enzymes possibly involved in sporulation and detoxification reactions
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