INSTITUTO DE FISIOLOGIA VEGETAL
Unidad Ejecutora - UE
Mapping and candidate gene identification of loci determining tolerance to greenbug (Schizaphis graminum, Rondani) in barley
TOCHO ÉRICA; BORNER ANDREAS; CASTRO ANA MARÍA
Lugar: Berlin; Año: 2013 vol. 191 p. 173 - 173
Greenbug is one of the most aggressivepests of barley and wheat. In Argentina, yield losses ofwheat, barley, oat and sorghum crops caused bygreenbug are chronic and at times severe. SinceMarker Assisted selection for greenbug resistancegenes in barley is very limited, the purpose of thecurrent study was to map greenbug resistance genes indoubled haploid (DH) lines and to identify candidategenes. A set of DH lines of the Oregon-Wolfe Barley(OWB) mapping population derived from the crossbetween OWBDOM and OWBREC and both parentallines were screened for tolerance to greenbug. Therewas significant variation among the DH lines in foliararea (FA), dry weight (DW) and chlorophyll contents(Ch) between infested and control DH lines. Threemain QTLs were identified. These QTLs explained82 % of the FA, 80 % of DW and 58 % of Chvariability of infested plants. The initial and final FAand DW of controls and final DW of infested plantswere associated with the same molecular markers onchromosome 2H (Vrs1, BmAc0144f, GBR259,GBS705). The final FA of infested plants was significantlylinked to molecular markers on chromosome5H (GBRO986, GBR518, GBM1483, GBR1082). Thepositive alleles were provided by OWBDOM. Thecontent of chlorophyll of infested plants was associatedwith the marker loci Ris44, GBR1608, GBR1637N andGBS0785 on chromosome 7H, with the positive allelesprovided by OWBREC. Both parents contributed todifferent tolerance traits. The QTLs found in thispopulation are new greenbug resistance loci. Asequence homology search was performed to derivethe putative function of the genes linked to the QTLs.