INFIVE   05416
INSTITUTO DE FISIOLOGIA VEGETAL
Unidad Ejecutora - UE
artículos
Título:
Validation of reference transcripts in strawberry (Fragaria spp.).
Autor/es:
CLANCY, MA; ROSLI HG; CHAMALA, S; BARBAZUK, W.B ; CIVELLO, PM; FOLTA K
Revista:
MOLECULAR GENETICS AND GENOMICS
Editorial:
SPRINGER HEIDELBERG
Referencias:
Lugar: HEIDELBERG; Año: 2013 vol. 288 p. 671 - 671
ISSN:
1617-4615
Resumen:
A set of strawberry (Fragaria spp.) constitutively-expressed reference genes has been identified by merging digital gene expression data with expression profiling. Constitutive reference candidates were validated using quantitative PCR and hybridization. Several transcripts have been identified that show improved stability across tissues relative to traditional controls such as actin or ubiquitin. Results are similar between commercial octoploid strawberry and the diploid model. Our findings also show that while some never-before-used references are appropriate for most applications, even the most stable reference transcripts require careful assessment across the diverse tissues and fruit developmental states before being adopted as controls.
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