INFIVE   05416
INSTITUTO DE FISIOLOGIA VEGETAL
Unidad Ejecutora - UE
artículos
Título:
Cladosporium cladosporioides LPSC 1088 produces the 1,8-dihydroxynaphthalene-melanin-like compound and carries a putative pks gene
Autor/es:
LLORENTE, C.; BÁRCENA, A.; VERA-BAHIMA, J.; MARIO CARLOS NAZARENO SAPARRAT; ARAMBARRI A. M.; FERNANDA ROZAS; MIRÍFICO, MARÍA VIRGINIA; BALATTI, P. A.
Revista:
MYCOPATHOLOGIA
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2012 vol. 174 p. 397 - 408
ISSN:
0301-486X
Resumen:
Cladosporium cladosporioides is a dematiaceous fungus with coloured
mycelia and conidia due to the presence of dark pigments. The purpose of
this study was to characterize the dark pigments synthetized by
Cladosporium sp. LPSC no. 1088 and also to identify the putative
polyketide synthase (pks) gene that might be involved in the pigment
biosynthesis. Morphological as well as molecular features like the ITS
sequence confirmed that LPSC 1088 is Cladosporium cladosporioides.
UV-visible, Fourier Transform Infrared (FTIR) and Electron Spin
Resonance (ESR) spectroscopy analysis as well as melanin inhibitors
suggest that the main dark pigment of the isolate was 1,8
dihydroxynaphthalene (DHN)-melanin-type compound. Two commercial
fungicides, Difenoconazole and Chlorothalonil, inhibited fungal growth
as well as increased pigmentation of the colonies suggesting that
melanin might protect the fungus against chemical stress. The pigment is
most probably synthetized by means of a pentaketide pathway since the
sequence of a 651 bp fragment, coding for a putative polyketide
synthase, is highly homologous to pks sequences from other fungi.